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Intracytoplasmic Sperm Injection (ICSI) for Infertile Couples

For couples facing challenges with infertility, Intracytoplasmic Sperm Injection (ICSI) offers a beacon of hope. This advanced medical procedure involves the direct injection of a single sperm into an egg, facilitating fertilization when conventional methods may have failed.

ICSI is particularly beneficial in cases of male infertility, such as low sperm count or motility issues, but it can also be employed when previous IVF attempts have been unsuccessful.

By providing a precise and targeted approach to fertilization, ICSI significantly improves the chances of conception, helping many couples realize their dreams of parenthood.

This page will explore the intricacies of the ICSI process, its benefits, and what couples can expect throughout their treatment journey.

The ICSI Process

ICS process at Pune IVF Fertility Centre

The ICSI process is a highly specialized technique that involves several critical steps to ensure the best chances of successful fertilization. 

Initially, the female partner undergoes ovarian stimulation, which is achieved through hormone injections to produce multiple mature eggs. These eggs are then retrieved from the ovaries using a minor surgical procedure under ultrasound guidance.


Once the eggs are collected, they are placed in a laboratory where embryologists prepare a single sperm for injection. This preparation often involves selecting the healthiest, most motile sperm from a semen sample. Using a microscopic needle, the embryologist carefully injects the selected sperm directly into the cytoplasm of each mature egg.

After the injection, the fertilized eggs, now known as embryos, are cultivated in a controlled environment for 3 to 5 days. During this period, the development of the embryos is closely monitored. Typically, the most viable embryo is chosen for transfer to the female partner’s uterus, optimizing the likelihood of implantation and pregnancy. Any remaining high-quality embryos can be cryopreserved for future use.

Throughout the entire ICSI journey, couples are supported by a multidisciplinary team of fertility specialists, ensuring they receive personalized care tailored to their unique needs. This meticulous and compassionate approach underpins the success of the ICSI process, bringing many couples one step closer to achieving their dream of starting a family.

At Pune IVF, the fertility experts are happy to answer your queries and guide your towards an informed choice if you are interested in ICSI treatment from a top IVF center in Pimple Saudagar, Pune.

Steps Involved in ICSI

The steps involved in the Intracytoplasmic Sperm Injection (ICSI) process are as follows:

Ovarian Stimulation

The female partner undergoes hormone injections to stimulate the ovaries to produce multiple mature eggs. This is monitored through regular blood tests and ultrasounds.

Egg Retrieval

Mature eggs are retrieved from the ovaries using a minor surgical procedure under ultrasound guidance, typically performed under mild sedation.

Sperm Selection

A semen sample is collected, and the healthiest, most motile sperm are selected for the injection process.

Sperm Injection

Using a microscopic needle, an embryologist carefully injects a single sperm directly into the cytoplasm of each mature egg.

Embryo Cultivation

The injected eggs, now fertilized and referred to as embryos, are cultured in a controlled environment for several days. Their development is closely monitored to identify the most viable embryos.

Embryo Transfer

The most viable embryo is selected for transfer to the female partner's uterus. This step is crucial for optimizing the chances of implantation and successful pregnancy.


Any remaining high-quality embryos are cryopreserved (frozen) for future use.

Support and Monitoring

Throughout the ICSI process, couples receive continuous support and personalized care from a team of fertility specialists, ensuring close monitoring and addressing any concerns to improve the chances of a successful outcome.

Advantages of ICSI

  1. Enhanced Fertilization Rates: ICSI significantly improves the chances of fertilization, especially in cases of severe male infertility or previous IVF failures.
  2. Broad Applicability: This technique can be used in a variety of scenarios, including low sperm count, low sperm motility, and unexplained infertility, making it a versatile option for many couples.
  3. Cryopreserved Sperm Utilization: ICSI allows for the effective use of cryopreserved sperm, which can be particularly beneficial for men who have stored sperm prior to medical treatments like chemotherapy.
  4. Donor Sperm and Eggs: Couples using donor sperm or eggs can also benefit from ICSI, as it increases the likelihood of successful fertilization with the high-quality reproductive materials.
  5. Controlled Fertilization Process: By injecting a single sperm directly into an egg, ICSI gives embryologists better control over the fertilization process, reducing the likelihood of fertilization failure.

Risks Involved with ICSI

Ovarian Hyperstimulation Syndrome (OHSS): Similar to conventional IVF, women undergoing ICSI are also at risk of OHSS, a condition where the ovaries respond too strongly to fertility drugs.

Multiple Pregnancies: The possibility of multiple pregnancies, which carry their own set of complications, remains a concern with ICSI, similar to other assisted reproductive techniques.

Emotional and Financial Strain: Couples should also consider the emotional and financial toll that ICSI can take, as the process can be stressful and expensive.

Success Rate

The success rate of in vitro ICSI can vary widely depending on numerous factors, including the age of the woman, the cause of infertility, and the quality of the IVF clinic. 

At an advanced dedicated IVF clinic with experienced IVF consultants success can reach as high as 70%

The success rate, however, significantly decreases as the woman’s age increases, with women over 40 experiencing a success rate closer to 30% per cycle. It’s important to note that these figures represent general statistics, and individual outcomes may differ.

The WHO also emphasises the importance of lifestyle factors, such as maintaining a healthy weight, abstaining from smoking, and managing stress, all of which can potentially improve IVF success rates. 

Additionally, selecting a clinic with high standards of care and experienced medical staff can make a significant difference.

By adhering to WHO guidelines and seeking comprehensive medical advice, prospective parents can better navigate the complexities of IVF and optimise their chances of success.

Average Cost Of IVF CYCLE WITH ICSI at Pune IVF

At Pune IVF, we believe in ethics and transparency as much as we believe in science and technology.

We do not believe in “one size fits all”

Every single patient is different and hence the protocol for each patient is tailor-made

On an  average, the cost ranges from ₹1,20,000 to ₹170,000 per cycle with self gametes.

This estimate typically covers consultations, medications, ultrasound monitoring, hormonal assays, egg retrieval, anaesthesia, embryologists fees, ICSI if required ,embryo freezing for 6 months and embryo transfer.

However, it may not include expenses for advanced procedures such as Laser assisted hatching, Preimplantation Genetic Testing (PGT-A), Hysteroscopy if required, which can add to the overall cost.

It is advisable for couples to get a detailed breakdown of fees from us to understand what is included and to plan their finances accordingly.

No cost EMI-

  • We help you plan your finances too
  • At Pune IVF, we believe in transparency and no pricing gimmicks. 
  • You pay what you have been counselled about. Because quality is priceless and so is your dream of parenthood
  • Hence we offer no cost EMI to our patients so that you do not have financial burden of treatment.
Please note- Pune IVF does not believe in giving multiple cycle  packages. We believe in quality work and strive hard to give you pregnancy in every single attempt.
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